1BP_PAIRWISE_KAKS(1) User Contributed Perl Documentation BP_PAIRWISE_KAKS(1)
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6 pairwise_kaks - script to calculate pairwise Ka,Ks for a set of
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10 pairwise_kaks.PLS -i t/data/worm_fam_2785.cdna [-f
11 fasta/genbank/embl...] [-msa tcoffee/clustal] [-kaks yn00/codeml]
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14 This script will take as input a dataset of cDNA sequences verify
15 that they contain no stop codons, align them in protein space,
16 project the alignment back into cDNA and estimate the Ka
17 (non-synonymous) and Ks (synonymous) substitutions based on the ML
18 method of Yang with the PAML package.
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20 Requires:
21 * bioperl-run package
22 * PAML program codeml or yn00
23 * Multiple sequence alignment programs Clustalw OR T-Coffee
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25 Often there are specific specific parameters you want to run when you
26 a computing Ka/Ks ratios so consider this script a starting point and
27 do not rely it on for every situation.
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30 Mailing Lists
31 User feedback is an integral part of the evolution of this and other
32 Bioperl modules. Send your comments and suggestions preferably to the
33 Bioperl mailing list. Your participation is much appreciated.
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35 bioperl-l@bioperl.org - General discussion
36 http://bioperl.org/wiki/Mailing_lists - About the mailing lists
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38 Reporting Bugs
39 Report bugs to the Bioperl bug tracking system to help us keep track of
40 the bugs and their resolution. Bug reports can be submitted via the
41 web:
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43 http://bugzilla.open-bio.org/
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46 Jason Stajich jason-at-bioperl-dot-org
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50perl v5.12.0 2010-04-29 BP_PAIRWISE_KAKS(1)