1samtools-rmdup(1) Bioinformatics tools samtools-rmdup(1)
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6 samtools rmdup - removes duplicate reads (obsolete)
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9 samtools rmdup [-sS] input.srt.bam out.bam
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13 This command is obsolete. Use markdup instead.
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15 Remove potential PCR duplicates: if multiple read pairs have identical
16 external coordinates, only retain the pair with highest mapping qual‐
17 ity. In the paired-end mode, this command ONLY works with FR orienta‐
18 tion and requires ISIZE is correctly set. It does not work for unpaired
19 reads (e.g. two ends mapped to different chromosomes or orphan reads).
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23 -s Remove duplicates for single-end reads. By default, the command
24 works for paired-end reads only.
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26 -S Treat paired-end reads and single-end reads.
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30 o Samtools paired-end rmdup does not work for unpaired reads (e.g. or‐
31 phan reads or ends mapped to different chromosomes). If this is a
32 concern, please use Picard's MarkDuplicates which correctly handles
33 these cases, although a little slower.
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37 Written by Heng Li from the Sanger Institute.
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41 samtools(1)
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43 Samtools website: <http://www.htslib.org/>
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47samtools-1.13 7 July 2021 samtools-rmdup(1)