1Ace::Sequence::GappedAlUisgenrmeCnotn(t3r)ibuted Perl DoAccuem:e:nSteaqtuieonnce::GappedAlignment(3)
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6 Ace::Sequence::GappedAlignment - Gapped alignment object
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9 # open database connection and get an Ace::Sequence object
10 use Ace::Sequence;
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12 # get a megabase from the middle of chromosome I
13 $seq = Ace::Sequence->new(-name => 'CHROMOSOME_I,
14 -db => $db,
15 -offset => 3_000_000,
16 -length => 1_000_000);
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18 # get all the gapped alignments
19 @alignments = $seq->alignments('EST_GENOME');
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21 # get the aligned segments from the first one
22 @segs = $alignments[0]->segments;
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24 # get the position of the first aligned segment on the
25 # source sequence:
26 ($s_start,$s_end) = ($segs[0]->start,$segs[0]->end);
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28 # get the target position for the first aligned segment
29 ($t_start,$t_end) = ($segs[0]->target->start,$segs[0]->target->end);
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32 Ace::Sequence::GappedAlignment is a subclass of Ace::Sequence::Feature.
33 It inherits all the methods of Ace::Sequence::Feature, but adds the
34 ability to retrieve the positions of the aligned segments. Each
35 segment is an Ace::Sequence::Feature, from which you can retrieve the
36 source and target coordinates.
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39 You will not ordinarily create an Ace::Sequence::GappedAlignment object
40 directly. Instead, objects will be created in response to a
41 alignments() call to an Ace::Sequence object.
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44 Most methods are inherited from Ace::Sequence::Feature. The following
45 methods are also supported:
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47 segments()
48 @segments = $gene->segments;
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50 Return a list of Ace::Sequence::Feature objects corresponding to
51 similar segments.
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53 relative()
54 $relative = $gene->relative;
55 $gene->relative(1);
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57 This turns on and off relative coordinates. By default, the exons
58 and intron features will be returned in the coordinate system used
59 by the gene. If relative() is set to a true value, then
60 coordinates will be expressed as relative to the start of the gene.
61 The first exon will (usually) be 1.
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64 Ace, Ace::Object, Ace::Sequence,Ace::Sequence::Homol,
65 Ace::Sequence::Feature, Ace::Sequence::FeatureList, GFF
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68 Lincoln Stein <lstein@cshl.org> with extensive help from Jean Thierry-
69 Mieg <mieg@kaa.crbm.cnrs-mop.fr>
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71 Copyright (c) 1999, Lincoln D. Stein
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73 This library is free software; you can redistribute it and/or modify it
74 under the same terms as Perl itself. See DISCLAIMER.txt for
75 disclaimers of warranty.
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78 Hey! The above document had some coding errors, which are explained
79 below:
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81 Around line 166:
82 You forgot a '=back' before '=head1'
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86perl v5.34.0 2022-01-20 Ace::Sequence::GappedAlignment(3)